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firststrand buffer solution  (Promega)

 
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    Structured Review

    Promega firststrand buffer solution
    Firststrand Buffer Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/firststrand+buffer+solution/first+strand+buffer/pm30639138-90-78-81
    Average 90 stars, based on 1 article reviews
    firststrand buffer solution - by Bioz Stars, 2026-10
    90/100 stars

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    Isolation:

    Article Title: Uremic solutes modulate hepatic bile acid handling and induce mitochondrial toxicity.
    Article Snippet: Next, efflux rates were normalized to the amount of cellular protein present in the samples as determined by a Biorad protein assay (Biorad). .. RNA was isolated from primary human hepatocytes exposed to 500 μM of uremic solutes or DMSO using the RNeasy® mini kit (Qiagen) following incubation at 37 °C and 5% (v/v) CO2 for 72 h. Subsequently, RNA was converted into cDNA using 300 ng of RNA, 0.625mM dNTPs (Life Technologies), 0.25 μg/μL random primers (Thermo Fisher Scientific), 10mM DTT (Promega), 20 U RNAsin (Promega), and 5% (v/ v) M-MLV (Thermo Fisher Scientific) in a total volume of 20 μL firststrand buffer solution (Promega), which was incubated at 20 °C for 10min, 42 °C for 45min, and 95 °C for 10min. .. Then, 2.5 μL of the resulting cDNA was mixed with 1.25 μL of primer-probe set (Applied Biosystems) for the transporter of interest, 12.5 μL Taqman mastermix (Applied Biosystems), and 8.75 μL of sterile MQ.

    Incubation:

    Article Title: Uremic solutes modulate hepatic bile acid handling and induce mitochondrial toxicity.
    Article Snippet: Next, efflux rates were normalized to the amount of cellular protein present in the samples as determined by a Biorad protein assay (Biorad). .. RNA was isolated from primary human hepatocytes exposed to 500 μM of uremic solutes or DMSO using the RNeasy® mini kit (Qiagen) following incubation at 37 °C and 5% (v/v) CO2 for 72 h. Subsequently, RNA was converted into cDNA using 300 ng of RNA, 0.625mM dNTPs (Life Technologies), 0.25 μg/μL random primers (Thermo Fisher Scientific), 10mM DTT (Promega), 20 U RNAsin (Promega), and 5% (v/ v) M-MLV (Thermo Fisher Scientific) in a total volume of 20 μL firststrand buffer solution (Promega), which was incubated at 20 °C for 10min, 42 °C for 45min, and 95 °C for 10min. .. Then, 2.5 μL of the resulting cDNA was mixed with 1.25 μL of primer-probe set (Applied Biosystems) for the transporter of interest, 12.5 μL Taqman mastermix (Applied Biosystems), and 8.75 μL of sterile MQ.



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    firststrand buffer solution  (Promega)
    90
    Promega firststrand buffer solution
    Firststrand Buffer Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/firststrand+buffer+solution/first+strand+buffer/pm30639138-90-78-81
    Average 90 stars, based on 1 article reviews
    firststrand buffer solution - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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